Functional analysis of tobacco LIM protein Ntlim1 involved in lignin biosynthesis

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Abstract

<jats:title>Summary</jats:title><jats:p>The AC‐rich motif, Pal‐box, is an important <jats:italic>cis</jats:italic>‐acting element for gene expression involved in phenylpropanoid biosynthesis. A cDNA clone (<jats:italic>Ntlim1</jats:italic>) encoding a Pal‐box binding protein was isolated by Southwestern screening. The deduced amino acid sequence is highly similar to the members of the LIM protein family that contain a zinc finger motif. Moreover, Ntlim1 had a specific DNA binding ability and transiently activated the transcription of a β‐glucuronidase reporter gene driven by the Pal‐box sequence in tobacco protoplasts. The transgenic tobacco plants with antisense Ntlim1 showed low levels of transcripts from some key phenylpropanoid pathway genes such as phenylalanine ammonia‐lyase, hydroxycinnamate CoA ligase and cinnamyl alcohol dehydrogenase. Furthermore, a 27% reduction of lignin content was observed in the transgenic tobacco with antisense <jats:italic>Ntlim1</jats:italic>.</jats:p>

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