<b>Regulation of a putative high‐affinity nitrate transporter (</b><i><b>Nrt2;1At</b></i><b>) in roots of</b><i><b>Arabidopsis thaliana</b></i>

• Degen Zhuo

• Mamoru Okamoto

• J. John Vidmar

• Anthony D. M. Glass

抄録

Putative high‐affinity nitrate (NO 3 – ) transporter genes, designated Nrt2;1At and Nrt2;2At , were isolated from Arabidopsis thaliana by RT–PCR using degenerate primers. The genes shared 86% and 89% identity at the amino acid and nucleotide levels, respectively, while their proteins shared 30–73% identities with other eukaryotic highaffinity NO 3 – transporters. Both genes were induced by NO 3 – , but Nrt2;1At gene expression was not apparent in 2‐ and 5‐day‐old plants. By 10 days, and thereafter, Nrt2;1At gene expression in roots was substantially higher than for the Nrt2;2At gene. Root Nrt2;1At expression levels were strongly correlated with inducible high‐affinity 13 NO 3 – influx into intact roots under several treatment conditions. The use of inhibitors of N assimilation indicated that downregulation of Nrt2;1At expression was mediated by NH 4 + , gln and other amino acids.

収録刊行物

• The Plant Journal

  The Plant Journal 17 (5), 563-568, 1999-03

  Wiley

キーワード

• Cell Biology
• Plant Science
• Genetics

詳細情報

• CRID: 1361418521425054208
• NII論文ID: 30014607372
• DOI: 10.1046/j.1365-313x.1999.00396.x
• ISSN: 1365313X
• ISSN: 09607412
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-313X.1999.00396.x
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-313X.1999.00396.x
• Web Site: https://onlinelibrary.wiley.com/doi/full-xml/10.1046/j.1365-313X.1999.00396.x
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