The <i>Arabidopsis</i> mutant <i>dct</i> is deficient in the plastidic glutamate/malate translocator DiT2

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<jats:title>Summary</jats:title><jats:p>The <jats:italic>Arabidopsis</jats:italic> mutant dicarboxylate transport (<jats:italic>dct</jats:italic>) is one of the classic mutants in the photorespiratory pathway. It requires high CO<jats:sub>2</jats:sub> levels for survival. Physiologic and biochemical characterization of <jats:italic>dct</jats:italic> indicated that <jats:italic>dct</jats:italic> is deficient in the transport of dicarboxylates across the chloroplast envelope membrane. Hence, re‐assimilation of ammonia generated by the photorespiratory cycle is blocked. However, the defective gene in <jats:italic>dct</jats:italic> has not been identified at the molecular level. Here, we report on the molecular characterization of the defective gene in <jats:italic>dct</jats:italic>, on the complementation of the mutant phenotype with a wild‐type cDNA, and on the functional characterization of the gene product, DiT2, in a recombinant reconstituted system. Furthermore, we provide the kinetic constants of recombinant DiT1 and DiT2, and we discuss these data with respect to their functions in ammonia assimilation. Moreover, an analysis of the transcript levels of DiT1 and DiT2 in different C<jats:sub>3</jats:sub>‐ and C<jats:sub>4</jats:sub>‐type plant species is presented, and we demonstrate that the substrate specificity of DiT2 from the C<jats:sub>4</jats:sub>‐plant <jats:italic>Flaveria bidentis</jats:italic> is similar to its counterpart from C<jats:sub>3</jats:sub> plants.</jats:p>

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