Role of cytosolic free calcium in the reorientation of pollen tube growth

• Rui Malhó

• Nick D. Read

• M. Salomé Pais

• Anthony J. Trewavas

抄録

Growing pollen tubes of Agapanthus umbellatus exhibited a tip‐to‐base gradient in cytosolic free calcium ([Ca 2+ ] c ). Although this gradient is believed to be involved in pollen tube growth, its role in specifying reorientation is unknown. The direction of pollen tube growth could be modified by iontophoretic micro‐injection, electrical fields (EFs) or photolysis of caged Ca 2+ . Iontophoretic injection resulted in a temporary cessation of growth, an increase in [Ca 2+ ] c and, upon recovery, reoriented growth. Weak EFs also elevated [Ca 2+ ] c , reduced growth rates and resulted in the reorientation of pollen tubes towards the cathode. Treatment with very low concentrations of the Ca 2+ ‐channel blocker lanthanum chloride, prior to exposure to an EF, inhibited both the increase in [Ca 2+ ] c and reorientation whilst only slightly affecting growth rates. The responses of growth inhibition and reorientation were mimicked when [Ca 2+ ] c was artificially elevated by photoactivating caged Ca 2+ (Nitr‐5). Our data suggest that [Ca 2+ ] c is part of a transduction mechanism which enables growing pollen tubes to successfully reorient to directional signals in the style and thus accomplish eventual fertilization of the egg.

収録刊行物

• The Plant Journal

  The Plant Journal 5 (3), 331-341, 1994-03

  Wiley

キーワード

• Cell Biology
• Plant Science
• Genetics

詳細情報

• CRID: 1363107368323161344
• NII論文ID: 30014609931
• DOI: 10.1111/j.1365-313x.1994.00331.x
• ISSN: 1365313X
• ISSN: 09607412
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-313X.1994.00331.x
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-313X.1994.00331.x
• データソース種別:
  • Crossref
  • CiNii Articles