Induction of gelatinolytic activities in ayu muscle at the spawning stage.

DOI Web Site 被引用文献6件 参考文献14件
  • KUBOTA SATOSHI
    Laboratory of Aquatic Product Utilization, Faculty of Agriculture, Kochi University
  • KINOSHITA MASATO
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
  • YOKOYAMA YOSHIHIRO
    Interdisciplinary Research Institute for Biosciences, Mukogawa Women's University
  • TOYOHARA HARUHIKO
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
  • SAKAGUCHI MORIHIKO
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University

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Gelatinolytic activities derived from both serine proteinase and metalloproteinase were induced in mature ayu muscle. These activities from ayu muscle at spawning stage (November) were fractionated successively by DEAE-cellulose, CM-cellulose, and gelatin affinity chromatographies, while no corresponding activity was detected from that of the growing stage (July). A gelatinolytic active band adsorbed to a CM-cellulose column was detected at 80 kDa by gelatin zymographic analysis. The activity was expressed at a slightly alkaline pH range and optimally at pH 8.5. The activity was inhibited by leupeptin and 1, 10-phenanthroline, but not by E-64, suggesting that it was composed of both serine proteinase and metalloproteinase. Serine-type activity at 80 kDa was not adsorbed to a gelatin-immobilized column. On the other hand, metallo-type activity was adsorbed to the column, and the molecular mass of the activity was estimated to be 68 kDa. Our findings suggested that these gelatinolytic activities due to both serine proteinase and metalloproteinase, which are specifically induced in the spawning stage, are possibly responsible for collagen breakdown according to maturation.

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