IL-12 protects mice against pulmonary and disseminated infection caused by <i>Cryptococcus neoformans</i>

• K Kawakami

  First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan

• M Tohyama

  First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan

• Q Xie

  First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan

• A Saito

  First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan

抄録

We examined the role of IL-12 in host resistance to Cryptococcus neoformans using a murine model of pulmonary and disseminated infection. In this model, mice were infected intratracheally with viable yeast cells. Mice untreated with IL-12 allowed an uncontrolled multiplication of yeast cells in the lung with infiltrations of few inflammatory cells, and a cryptococcal dissemination to the brain and meningitis by 3 weeks, resulting in death of all animals within 4–6 weeks. IL-12, when administered from the day of tracheal infection for 7 days, induced a marked infiltration of inflammatory cells, consisting mostly of mononuclear cells, and significantly reduced the number of viable yeast cells in the lung. The treatment suppressed brain dissemination, as shown by a marked reduction of yeast cells in the brain and prevention of meningitis. These effects resulted in a significant increase in the survival rate of infected mice. In contrast, late administration of IL-12 commencing on day 7 after instillation of yeast cells failed to protect the mice against infection with C. neoformans. In further experiments, early administration of IL-12 markedly induced interferon-gamma (IFN-γ) mRNA in the lungs of infected mice, while no IFN-γ mRNA was detected without this treatment. Our results indicate that IL-12 is effective when administered in the early period of pulmonary cryptococcal infection.

収録刊行物

• Clinical and Experimental Immunology

  Clinical and Experimental Immunology 104 (2), 208-214, 1996-05

  Oxford University Press (OUP)

キーワード

• Immunology
• Immunology and Allergy

詳細情報

• CRID: 1363951795067640320
• NII論文ID: 30015017722
• DOI: 10.1046/j.1365-2249.1996.14723.x
• ISSN: 13652249
• ISSN: 00099104
• ISSN: http://id.crossref.org/issn/00099104
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2249.1996.14723.x
• Web Site: https://academic.oup.com/cei/article-pdf/104/2/208/42203798/j.1365-2249.1996.14723.x.pdf
• データソース種別:
  • Crossref
  • CiNii Articles