A cortical granule‐specific enzyme, B‐1,3‐glucanase, in sea urchin eggs
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<jats:title>Abstract</jats:title><jats:p>The ultrastructural localization of B‐1,3‐glucanase in three species of sea urchin eggs was determined using a monospecific antibody in an electronmicroscopic immunogold procedure. In all three species, <jats:italic>Lytechinus variegatus, Strongylocentrotus purpuratus</jats:italic>, and <jats:italic>Arbacia punctulata</jats:italic>, B‐1,3‐glucanase was localized specifically to the cortical granules. No other organelle within the egg contained significant label. During the fertilization reaction, B‐1,3‐glucanase was released from cortical granules into the perivitelline space and became associated with the hyaline layer. No significant label was found in association with the fertilization envelope.</jats:p>
収録刊行物
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- Gamete Research
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Gamete Research 18 (4), 339-348, 1987-12
Wiley
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詳細情報
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- CRID
- 1361418520600289280
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- NII論文ID
- 30015914230
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- NII書誌ID
- AA00653134
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- ISSN
- 15543919
- 01487280
- http://id.crossref.org/issn/01487280
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- データソース種別
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- Crossref
- CiNii Articles