Feasibility of photoexcitation imaging in soft x-ray microscopy using the transition to π* orbits in biological molecules

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  • Yoshinori Iketaki
    ‘‘Light and Material’’ PRESTO, Research Development Corporation of Japan, 2-45-13 Honcho, Nakano-ku, Tokyo 164, Japan
  • Tsutomu Watanabe
    Department of Physics, International Christian University, 3-10-2 Ohsawa, Mitaka-shi, Tokyo 181, Japan

Abstract

<jats:p>In the so-called water-window wavelength region, the photoexcitation cross section is found to be larger by two orders of magnitude than those for the photoionizations of atoms. Making use of these physical phenomena, a new imaging method for microscopy was studied. We have concluded that this method is useful for the measuring the molecules in DNA or RNA of wet biological cells. A sufficient contrast for microscopy measurements can be obtained by using the photoexcitation of the 1s-orbital electron to π* orbits.</jats:p>

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