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- Eric Johannsen
- Program in Virology, Departments of Microbiology and Molecular Genetics and Medicine, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115; and Harvard Medical School and Partners HealthCare Center for Genetics and Genomics, Cambridge, MA 02139
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- Micah Luftig
- Program in Virology, Departments of Microbiology and Molecular Genetics and Medicine, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115; and Harvard Medical School and Partners HealthCare Center for Genetics and Genomics, Cambridge, MA 02139
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- Michael R. Chase
- Program in Virology, Departments of Microbiology and Molecular Genetics and Medicine, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115; and Harvard Medical School and Partners HealthCare Center for Genetics and Genomics, Cambridge, MA 02139
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- Steve Weicksel
- Program in Virology, Departments of Microbiology and Molecular Genetics and Medicine, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115; and Harvard Medical School and Partners HealthCare Center for Genetics and Genomics, Cambridge, MA 02139
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- Ellen Cahir-McFarland
- Program in Virology, Departments of Microbiology and Molecular Genetics and Medicine, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115; and Harvard Medical School and Partners HealthCare Center for Genetics and Genomics, Cambridge, MA 02139
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- Diego Illanes
- Program in Virology, Departments of Microbiology and Molecular Genetics and Medicine, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115; and Harvard Medical School and Partners HealthCare Center for Genetics and Genomics, Cambridge, MA 02139
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- David Sarracino
- Program in Virology, Departments of Microbiology and Molecular Genetics and Medicine, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115; and Harvard Medical School and Partners HealthCare Center for Genetics and Genomics, Cambridge, MA 02139
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- Elliott Kieff
- Program in Virology, Departments of Microbiology and Molecular Genetics and Medicine, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115; and Harvard Medical School and Partners HealthCare Center for Genetics and Genomics, Cambridge, MA 02139
抄録
<jats:p>Mature Epstein-Barr virus (EBV) was purified from the culture medium of infected lymphocytes made functionally conditional for Zta activation of lytic replication by an in-frame fusion with a mutant estrogen receptor. Proteins in purified virus preparations were separated by gradient gel electrophoresis and trypsin-digested; peptides were then analyzed by tandem hydrophobic chromatography, tandem MS sequencing, and MS scans. Potential peptides were matched with EBV and human gene ORFs. Mature EBV was mostly composed of homologues of proteins previously found in a herpes virion. However, EBV homologues to herpes simplex virus capsid-associated or tegument components UL7 (BBRF2), UL14 (BGLF3), and EBV BFRF1 were not significantly detected. Instead, probable tegument components included the EBV and γ-herpesvirus-encoded BLRF2, BRRF2, BDLF2 and BKRF4 proteins. Actin was also a major tegument protein, and cofilin, tubulin, heat shock protein 90, and heat shock protein 70 were substantial components. EBV envelope glycoprotein gp350 was highly abundant, followed by glycoprotein gH, intact and furin-cleaved gB, gM, gp42, gL, gp78, gp150, and gN. BILF1 (gp64) and proteins associated with latent EBV infection were not detected in virions.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 101 (46), 16286-16291, 2004-11-08
Proceedings of the National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1362825894937167744
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- NII論文ID
- 30016244394
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- ISSN
- 10916490
- 00278424
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- データソース種別
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