Iron release from macrophages after erythrophagocytosis is up-regulated by ferroportin 1 overexpression and down-regulated by hepcidin

• Mitchell D. Knutson

  Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611; and Departments of Immunology and Infectious Diseases and Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115

• Mohamed Oukka

  Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611; and Departments of Immunology and Infectious Diseases and Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115

• Lindsey M. Koss

  Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611; and Departments of Immunology and Infectious Diseases and Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115

• Fikret Aydemir

  Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611; and Departments of Immunology and Infectious Diseases and Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115

• Marianne Wessling-Resnick

  Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611; and Departments of Immunology and Infectious Diseases and Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115

抄録

Ferroportin 1 (FPN1) is transmembrane protein involved in iron homeostasis. In the duodenum, FPN1 localizes to the basolateral surface of enterocytes where it appears to export iron out of the cell and into the portal circulation. FPN1 is also abundantly expressed in reticuloendothelial macrophages of the liver, spleen, and bone marrow, suggesting that this protein serves as an iron exporter in cells that recycle iron from senescent red blood cells. To directly test the hypothesis that FPN1 functions in the export of iron after erythrophagocytosis, FPN1 was stably expressed in J774 mouse macrophages by using retroviral transduction, and release of 59 Fe after phagocytosis of 59 Fe-labeled rat erythrocytes was measured. J774 cells overexpressing FPN1 released 70% more 59 Fe after erythrophagocytosis than control cells, consistent with a role in the recycling of iron from senescent red cells. Treatment of cells with the peptide hormone hepcidin, a systemic regulator of iron metabolism, dramatically decreased FPN1 protein levels and significantly reduced the efflux of 59 Fe after erythrophagocytosis. Subsequent fractionation of the total released 59 Fe into heme and nonheme compounds revealed that hepcidin treatment reduced the release of nonheme 59 Fe by 50% and 25% from control and FPN1-overexpressing cells, respectively, but did not diminish efflux of 59 Fe-heme. We conclude that FPN1 is directly involved in the export of iron during erythrocyte-iron recycling by macrophages.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 102 (5), 1324-1328, 2005-01-21

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1361981470059755776
• NII論文ID: 30016245026
• DOI: 10.1073/pnas.0409409102
• ISSN: 10916490
• ISSN: 00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.0409409102
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