A Novel Closed-Circular Mitochondrial DNA with Properties of a Replicating Intermediate

  • Harumi Kasamatsu
    Division of Biology, California Institute of Technology, Pasadena, Calif. 91109
  • Donald L. Robberson
    Division of Biology, California Institute of Technology, Pasadena, Calif. 91109
  • Jerome Vinograd
    Division of Biology, California Institute of Technology, Pasadena, Calif. 91109

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<jats:p>A fraction of the covalently closed mitochondrial DNA in mouse L cells contains a replicated heavy-strand segment that is hydrogen bonded to the circular light strand. The inserted single strand is dissociable from the circular duplex at an elevated temperature.</jats:p>

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