Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose
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- Haim Aviv
- Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20014
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- Philip Leder
- Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20014
Abstract
<jats:p>A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active rabbit globin mRNA has been purified by this procedure and assayed for its ability to direct the synthesis of rabbit globin in a cell-free extract of ascites tumor. Inasmuch as various mammalian mRNAs appear to be rich in poly(adenylic acid) and can likely be translated in the ascites cell-free extract, this approach should prove generally useful as an initial step in the isolation of specific mRNAs.</jats:p>
Journal
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 69 (6), 1408-1412, 1972-06
Proceedings of the National Academy of Sciences
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Keywords
Details 詳細情報について
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- CRID
- 1362262945657215872
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- NII Article ID
- 30016259534
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- ISSN
- 10916490
- 00278424
- http://id.crossref.org/issn/00278424
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- Data Source
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- Crossref
- CiNii Articles