DNA sequencing with chain-terminating inhibitors

• F. Sanger

  Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, England

• S. Nicklen

  Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, England

• A. R. Coulson

  Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 2QH, England

抄録

A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage ϕX174 and is more rapid and more accurate than either the plus or the minus method.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 74 (12), 5463-5467, 1977-12

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1360011145972573824
• NII論文ID: 30016264931
• DOI: 10.1073/pnas.74.12.5463
• ISSN: 10916490
• ISSN: 00278424
• ISSN: http://id.crossref.org/issn/00278424
• ISSN: http://id.crossref.org/issn/03859037
• ISSN: http://id.crossref.org/issn/07644450
• ISSN: http://id.crossref.org/issn/0015749X
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.74.12.5463
• データソース種別:
  • Crossref
  • CiNii Articles