Appearance of catecholamine-synthesizing enzymes during development of rat sympathetic nervous system: Possible role of tissue environment
-
- Gladys Teitelman
- Laboratory of Neurobiology, Department of Neurology, Cornell University Medical College, New York, New York 10021
-
- Harriet Baker
- Laboratory of Neurobiology, Department of Neurology, Cornell University Medical College, New York, New York 10021
-
- Tong H. Joh
- Laboratory of Neurobiology, Department of Neurology, Cornell University Medical College, New York, New York 10021
-
- Donald J. Reis
- Laboratory of Neurobiology, Department of Neurology, Cornell University Medical College, New York, New York 10021
抄録
<jats:p> We sought to determine, in rat embryo, when and at what site in their migration cells derived from the neural crest differentiate into sympathetic neuroblasts. This has been accomplished by immunocytochemical detection, within the cells, of the enzymes catalyzing catecholamine biosynthesis—tyrosine hydroxylase [TH; tyrosine 3-monooxygenase, L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2] dopamine-β-hydroxylase [DBH; 3,4-dihydroxyphenylethylamine,ascorbate:oxygen oxidoreductase (β-hydroxylating), EC 1.14.17.1)]—and, as a marker of prospective adrenal medullary cells, the enzyme phenylethanolamine <jats:italic>N</jats:italic> -methyltransferase (PNMT; <jats:italic>S</jats:italic> -adenosyl-L-methionine:phenylethanolamine <jats:italic>N</jats:italic> -methyltransferase, EC 2.1.1.28). TH and DBH, not detected in the neural crest, appear almost simultaneously in cells of the thoracic sympathetic ganglia in 11-day-old embryos, and in abdominal and lumbar ganglia 1-2 days later, thereby exhibiting a characteristic rostral-caudal gradient of differentiation. Cells stained for TH and DBH are seen in the gut wall from day 11 to day 14, but not thereafter. Cells stained for TH and DBH appear in the adrenal anlage at day 15. However, PNMT is not detected in the adrenal until day 17 of development, and is present only in the sympathoblasts in contact with the adrenal cortex. Treatment of pregnant rats with dexamethasone failed to accelerate the appearance of PNMT in the embryo or to initiate its expression in cells of other sympathetic organs. We conclude that neural crest cells express a noradrenergic phenotype only after leaving the neural crest and that these cells are labile with respect to their neurotransmitter and are capable of transformation in response to environmental stimuli. </jats:p>
収録刊行物
-
- Proceedings of the National Academy of Sciences
-
Proceedings of the National Academy of Sciences 76 (1), 509-513, 1979-01
Proceedings of the National Academy of Sciences
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1361418521293259776
-
- NII論文ID
- 30016267767
-
- ISSN
- 10916490
- 00278424
- http://id.crossref.org/issn/00278424
-
- データソース種別
-
- Crossref
- CiNii Articles