Coordinate gene expression during somatic embryogenesis in carrots

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  • Z. R. Sung
    Department of Genetics, University of California, Berkeley, California 94720
  • R. Okimoto
    Department of Genetics, University of California, Berkeley, California 94720

Abstract

<jats:p> There are several biochemical differences between the callus and the embryos of carrot culture. Callus tissue produces callus-specific proteins and a conditioning factor that is necessary for the synthesis of callus-specific proteins. By contrast, embryos produce embryo-specific proteins [Sung, Z. R. & Okimoto, R. (1981) <jats:italic>Proc. Natl. Acad. Sci. USA</jats:italic> 78, 3683-3687] and develop the capability to inactivate cycloheximide [Sung, Z. R., Lazar, G. J. & Dudits, D. (1981) <jats:italic>Plant Physiol</jats:italic> . 68, 261-264]. A mutant, WCH105, that can inactivate cycloheximide in the callus as well as in the embryos produces the embryo-specific proteins instead of the callus-specific proteins and fails to produce the conditioning factor by the callus tissue. Callus tissues also produce a conditioning factor for callus growth. This factor is not the same as the conditioning factor for the synthesis of the callus-specific proteins, as WCH105 can grow as callus. The existence of WCH105 demonstrates that the callus-specific and embryo-specific traits are coordinately regulated, but in an opposite manner. A common mechanism apparently activates one set and inactivates the other set of functions. WCH105 seems to be impaired in this mechanism. </jats:p>

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