Developmental changes in the expression and compartmentalization of the glucocorticoid receptor in embryonic retina.

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  • R Gorovits
    Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
  • I Ben-Dror
    Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
  • L E Fox
    Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
  • H M Westphal
    Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
  • L Vardimon
    Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.

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<jats:p>Inducibility by glucocorticoids of the glutamine synthetase gene in chicken embryo retina and the transcriptional activity of the glucocorticoid receptor (GR) greatly increase between embryonic days 6 and 10 (E6, E10), although the level of GR does not markedly change during that time. This apparent discrepancy was investigated by examining the pattern of GR expression in undifferentiated E6 retina and in E10 retina, which consists mostly of differentiated cells. Two GR isoforms, 90 and 95 kDa, were found to be expressed at both of these ages at a similar total level but in different proportions: in E6 retina the level of the 90-kDa isoform was higher, whereas in E10 retina the 95-kDa receptor was higher. However, following treatment of the retinas with cortisol, the 95-kDa isoform became the predominant receptor at both ages. Immunohistochemical analysis revealed that the cellular localization of GR markedly changed in the course of development: in the undifferentiated E6 retina GR was expressed in virtually all cells, whereas in the more differentiated E10 and E12 retina, GR was detected only in Müller glia cells. The latter represent approximately 20% of the cells in this tissue and are the only cells in which glucocorticoid hormone induces the glutamine synthetase gene. We suggest that the compartmentalization of GR in Müller glia is a major aspect of the mechanism that modulates receptor activity during retina development and results in the temporal increase in the inducibility of glutamine synthetase and its specific localization in Müller glia cells.</jats:p>

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