Solubilization of beta-amyloid-(1-42)-peptide: reversing the beta-sheet conformation induced by aluminum with silicates.
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- G D Fasman
- Graduate Department of Biochemistry, Brandeis University, Waltham, MA 02254.
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- A Perczel
- Graduate Department of Biochemistry, Brandeis University, Waltham, MA 02254.
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- C D Moore
- Graduate Department of Biochemistry, Brandeis University, Waltham, MA 02254.
抄録
<jats:p>Plaques are one of the two lesions found in the brain of patients with Alzheimer disease. Using a synthetic peptide corresponding to rat beta-amyloid-(1-42) (beta A4), circular dichroism (CD) analyses were performed to examine the effect of Na4SiO4 on the conformational state produced by Al3+. A previous study on fragments of neuronal proteins involved in tangle formation had shown a conformational transition from a beta-pleated sheet to a soluble random coil upon addition of Na4SiO4. In the present study, CD measurements showed that the beta-pleated sheet conformation of beta A4 induced by Al3+ was reversed to the random coil soluble form by the addition of Na4SiO4. The tight binding of SiO4(4-) with Al3+ provides the mechanism for this transition. These results provide insight into the role of aluminum in the Alzheimer diseased brain and suggests that investigation of the use of silicates as a therapeutic agent.</jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 92 (2), 369-371, 1995-01-17
Proceedings of the National Academy of Sciences
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詳細情報 詳細情報について
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- CRID
- 1364233269097208832
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- NII論文ID
- 30016299963
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- ISSN
- 10916490
- 00278424
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