Aurora A phosphorylation of TACC3/maskin is required for centrosome-dependent microtubule assembly in mitosis

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  • Kazuhisa Kinoshita
    1Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), 01307 Dresden, Germany
  • Tim L. Noetzel
    1Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), 01307 Dresden, Germany
  • Laurence Pelletier
    1Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), 01307 Dresden, Germany
  • Karl Mechtler
    2Research Institute of Molecular Pathology, A-1030 Vienna, Austria
  • David N. Drechsel
    1Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), 01307 Dresden, Germany
  • Anne Schwager
    1Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), 01307 Dresden, Germany
  • Mike Lee
    3The Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge CB2 1QN, England, UK
  • Jordan W. Raff
    3The Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge CB2 1QN, England, UK
  • Anthony A. Hyman
    1Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), 01307 Dresden, Germany

抄録

<jats:p>Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3–XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A–dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis.</jats:p>

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