Expression of the <i>Elm1</i> Gene, a Novel Gene of the CCN (Connective Tissue Growth Factor, Cyr61/Cef10, and Neuroblastoma Overexpressed Gene) Family, Suppresses In Vivo Tumor Growth and Metastasis of K-1735 Murine Melanoma Cells
-
- Yasunobu Hashimoto
- From the *Biology Division, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104, Japan; the ‡Department of Urology, Tokyo Women's Medical College, Kawadacho 8-chome, Shinjyuku-ku, Tokyo 162, Japan; and the §Department of Mammalian Genetics Laboratory, National Institute of Genetics, 1-111, Tanida, Mishima, Shizuoka 411, Japan
-
- Nobuko Shindo-Okada
- From the *Biology Division, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104, Japan; the ‡Department of Urology, Tokyo Women's Medical College, Kawadacho 8-chome, Shinjyuku-ku, Tokyo 162, Japan; and the §Department of Mammalian Genetics Laboratory, National Institute of Genetics, 1-111, Tanida, Mishima, Shizuoka 411, Japan
-
- Masachika Tani
- From the *Biology Division, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104, Japan; the ‡Department of Urology, Tokyo Women's Medical College, Kawadacho 8-chome, Shinjyuku-ku, Tokyo 162, Japan; and the §Department of Mammalian Genetics Laboratory, National Institute of Genetics, 1-111, Tanida, Mishima, Shizuoka 411, Japan
-
- Yasuhiro Nagamachi
- From the *Biology Division, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104, Japan; the ‡Department of Urology, Tokyo Women's Medical College, Kawadacho 8-chome, Shinjyuku-ku, Tokyo 162, Japan; and the §Department of Mammalian Genetics Laboratory, National Institute of Genetics, 1-111, Tanida, Mishima, Shizuoka 411, Japan
-
- Kaori Takeuchi
- From the *Biology Division, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104, Japan; the ‡Department of Urology, Tokyo Women's Medical College, Kawadacho 8-chome, Shinjyuku-ku, Tokyo 162, Japan; and the §Department of Mammalian Genetics Laboratory, National Institute of Genetics, 1-111, Tanida, Mishima, Shizuoka 411, Japan
-
- Toshihiko Shiroishi
- From the *Biology Division, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104, Japan; the ‡Department of Urology, Tokyo Women's Medical College, Kawadacho 8-chome, Shinjyuku-ku, Tokyo 162, Japan; and the §Department of Mammalian Genetics Laboratory, National Institute of Genetics, 1-111, Tanida, Mishima, Shizuoka 411, Japan
-
- Hiroshi Toma
- From the *Biology Division, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104, Japan; the ‡Department of Urology, Tokyo Women's Medical College, Kawadacho 8-chome, Shinjyuku-ku, Tokyo 162, Japan; and the §Department of Mammalian Genetics Laboratory, National Institute of Genetics, 1-111, Tanida, Mishima, Shizuoka 411, Japan
-
- Jun Yokota
- From the *Biology Division, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo 104, Japan; the ‡Department of Urology, Tokyo Women's Medical College, Kawadacho 8-chome, Shinjyuku-ku, Tokyo 162, Japan; and the §Department of Mammalian Genetics Laboratory, National Institute of Genetics, 1-111, Tanida, Mishima, Shizuoka 411, Japan
この論文をさがす
抄録
<jats:p>We previously isolated a partial cDNA fragment of a novel gene, Elm1 (expressed in low-metastatic cells), that is expressed in low-metastatic but not in high-metastatic K-1735 mouse melanoma cells. Here we determined the full-length cDNA structure of Elm1 and investigated the effect of Elm1 expression on growth and metastatic potential of K-1735 cells. The Elm1 gene encodes a predicted protein of 367 amino acids showing ∼40% amino acid identity with the CCN (connective tissue growth factor [CTGF], Cyr61/Cef10, neuroblastoma overexpressed gene [Nov]) family proteins, which consist of secreted cysteine-rich proteins with growth regulatory functions. Elm1 is also a cysteine-rich protein and contains a signal peptide and four domains conserved in the CCN family proteins. Elm1 was highly conserved, expressed ubiquitously in diverse organs, and mapped to mouse chromosome 15. High-metastatic K-1735 M-2 cells, which did not express Elm1, were transfected with an Elm1 expression vector, and several stable clones with Elm1 expression were established. The in vivo growth rates of cells expressing a high level of Elm1 were remarkably slower than those of cells expressing a low level of Elm1. Metastatic potential of transfectants was reduced in proportion to the level of Elm1 expression. Thus, Elm1 is a novel gene of CCN family that can suppress the in vivo growth and metastatic potential of K-1735 mouse melanoma cells.</jats:p>
収録刊行物
-
- The Journal of Experimental Medicine
-
The Journal of Experimental Medicine 187 (3), 289-296, 1998-02-02
Rockefeller University Press
- Tweet
詳細情報
-
- CRID
- 1363951793646789120
-
- NII論文ID
- 30017416252
-
- NII書誌ID
- AA00697559
-
- ISSN
- 15409538
- 00221007
-
- データソース種別
-
- Crossref
- CiNii Articles