Normal B Cell Homeostasis Requires B Cell Activation Factor Production by Radiation-resistant Cells
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- Leonid Gorelik
- Biogen Inc., Cambridge, MA 02142
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- Kevin Gilbride
- Biogen Inc., Cambridge, MA 02142
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- Max Dobles
- Biogen Inc., Cambridge, MA 02142
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- Susan L. Kalled
- Biogen Inc., Cambridge, MA 02142
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- Daniel Zandman
- Biogen Inc., Cambridge, MA 02142
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- Martin L. Scott
- Biogen Inc., Cambridge, MA 02142
抄録
<jats:p>The cellular source of B cell activation factor (BAFF) required for peripheral B cell survival/maturation is unknown. To determine the nature of BAFF-producing cells we established and analyzed reciprocal bone marrow (BM) chimeras with wild-type (WT) and BAFF-deficient mice. The results revealed that BAFF production by radiation-resistant stromal cells is completely sufficient to provide a necessary signal for B cell survival/maturation, as BAFF−/− BM cells transferred into lethally irradiated WT mice gave rise to normal numbers of follicular (FO) and marginal zone (MZ) B cell subpopulations. On the other hand, transfer of WT BM into BAFF−/− lethally irradiated mice resulted only in minimal reconstitution of mature FO B cells and no restoration of MZ B cells. Thus, in the absence of BAFF+/+ stromal cells, BAFF production by BM-derived cells, presumably by macrophages, dendritic cells, and/or neutrophils, was not at all sufficient to support normal B cell homeostasis. Interestingly, immunization of both types of chimeras stimulated high levels of antigen-specific antibody secretion, indicating that either stromal cell– or hematopoietic cell–derived BAFF is sufficient for B cell antibody responses.</jats:p>
収録刊行物
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- The Journal of Experimental Medicine
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The Journal of Experimental Medicine 198 (6), 937-945, 2003-09-15
Rockefeller University Press
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詳細情報 詳細情報について
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- CRID
- 1363670320949001472
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- NII論文ID
- 30017417236
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- ISSN
- 15409538
- 00221007
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- データソース種別
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