Analysis of interleukin 6 receptor and gp130 expressions and proliferative capability of human CD34+ cells.
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- S Tajima
- Department of Clinical Oncology, University of Tokyo, Japan.
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- K Tsuji
- Department of Clinical Oncology, University of Tokyo, Japan.
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- Y Ebihara
- Department of Clinical Oncology, University of Tokyo, Japan.
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- X Sui
- Department of Clinical Oncology, University of Tokyo, Japan.
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- R Tanaka
- Department of Clinical Oncology, University of Tokyo, Japan.
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- K Muraoka
- Department of Clinical Oncology, University of Tokyo, Japan.
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- M Yoshida
- Department of Clinical Oncology, University of Tokyo, Japan.
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- K Yamada
- Department of Clinical Oncology, University of Tokyo, Japan.
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- K Yasukawa
- Department of Clinical Oncology, University of Tokyo, Japan.
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- T Taga
- Department of Clinical Oncology, University of Tokyo, Japan.
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- T Kishimoto
- Department of Clinical Oncology, University of Tokyo, Japan.
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- T Nakahata
- Department of Clinical Oncology, University of Tokyo, Japan.
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Abstract
<jats:p>We recently demonstrated that stimulation of gp130 by a combination of soluble interleukin 6 receptor (sIL-6R) and IL-6 but not IL-6 alone significantly stimulates the ex vivo expansion of primitive hematopoietic progenitors and the generation of erythroid cells from human CD34+ cells in the presence of stem cell factor (SCF). Here, we show that gp130 is found low positively on most CD34+ cells, whereas IL-6R is expressed on only 30-50% of these cells. Although most of the colonies generated from FACS-sorted CD34+IL-6R+ cells were granulocyte/macrophage (GM) colonies, CD34+IL-6R- cells gave rise to various types of colonies, including erythroid bursts, GM, megakaryocytes, and mixed colonies in methylcellulose culture with a combination of IL-6, sIL-6R, and SCF. Similar results were obtained in culture supplemented with a combination of IL-3, IL-6, SCF, granulocyte colony-stimulating factor, erythropoietin, and thrombopoietin. A limiting dilution analysis of long-term culture-initiating cells (LTC-IC) showed that the CD34+IL-6R- cells contained a larger number of LTC-IC than did the CD34+IL-6R+ cells. In a serum-free suspension of CD34+IL-6R- cells, the addition of sIL-6R to the combination of IL-6 and SCF dramatically increased the total and multipotential progenitors, whereas CD34+IL-6R+ cells failed to do so under the same conditions. These results indicate that most of the erythroid, megakaryocytic, and primitive human hematopoietic progenitors are included in the IL-6R- populations, and the activation of gp130 on these progenitors can be achieved by a complex of IL-6-sIL-6R, but not by IL-6 alone. The present culture system using IL-6, sIL-6R, and SCF may provide a novel approach for ex vivo expansion of human primitive hematopoietic progenitors.</jats:p>
Journal
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- The Journal of experimental medicine
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The Journal of experimental medicine 184 (4), 1357-1364, 1996-10-01
Rockefeller University Press
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Keywords
Details 詳細情報について
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- CRID
- 1360855569941187584
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- NII Article ID
- 30017431265
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- NII Book ID
- AA00697559
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- ISSN
- 15409538
- 00221007
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- Data Source
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- Crossref
- CiNii Articles