Spindle Formation in<i>Aspergillus</i>Is Coupled to Tubulin Movement into the Nucleus

• Yulia Ovechkina

  Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210

• Paul Maddox

  Department of Biology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina 27599

• C. Elizabeth Oakley

  Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210

• Xin Xiang

  Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814

• Stephen A. Osmani

  Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210

• Edward D. Salmon

  Department of Biology, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina 27599

• Berl R. Oakley

  Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210

抄録

In many important organisms, including many algae and most fungi, the nuclear envelope does not disassemble during mitosis. This fact raises the possibility that mitotic onset and/or exit might be regulated, in part, by movement of important mitotic proteins into and out of the nucleoplasm. We have used two methods to determine whether tubulin levels in the nucleoplasm are regulated in the fungus Aspergillus nidulans. First, we have used benomyl to disassemble microtubules and create a pool of free tubulin that can be readily observed by immunofluorescence. We find that tubulin is substantially excluded from interphase nuclei, but is present in mitotic nuclei. Second, we have observed a green fluorescent protein/α-tubulin fusion in living cells by time-lapse spinning-disk confocal microscopy. We find that tubulin is excluded from interphase nuclei, enters the nucleus seconds before the mitotic spindle begins to form, and is removed from the nucleoplasm during the M-to-G 1 transition. Our data indicate that regulation of intranuclear tubulin levels plays an important, perhaps essential, role in the control of mitotic spindle formation in A. nidulans. They suggest that regulation of protein movement into the nucleoplasm may be important for regulating mitotic onset in organisms with intranuclear mitosis.

収録刊行物

• Molecular Biology of the Cell

  Molecular Biology of the Cell 14 (5), 2192-2200, 2003-05

  American Society for Cell Biology (ASCB)

キーワード

• Cell Biology
• Molecular Biology

詳細情報

• CRID: 1361137044820171136
• NII論文ID: 30018378434
• DOI: 10.1091/mbc.e02-10-0641
• ISSN: 19394586
• ISSN: 10591524
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