The c<sub>15</sub> ring of the <i>Spirulina platensis</i> F‐ATP synthase: F<sub>1</sub>/F<sub>0</sub> symmetry mismatch is not obligatory

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  • Denys Pogoryelov
    Institute of Microbiology, Eidgenössische Technische Hochschule Wolfgang‐Pauli Strasse 10 Zurich 8093 Switzerland
  • Jinshu Yu
    Department of Cellular Machines, Centre for Biotechnology, University of Technology Tatzberg 47 Dresden 01307 Germany
  • Thomas Meier
    Institute of Microbiology, Eidgenössische Technische Hochschule Wolfgang‐Pauli Strasse 10 Zurich 8093 Switzerland
  • Janet Vonck
    Department of Structural Biology, Max Planck Institute of Biophysics Max‐von‐Laue Strasse 3, Frankfurt am Main 60438 Germany
  • Peter Dimroth
    Institute of Microbiology, Eidgenössische Technische Hochschule Wolfgang‐Pauli Strasse 10 Zurich 8093 Switzerland
  • Daniel J Muller
    Department of Cellular Machines, Centre for Biotechnology, University of Technology Tatzberg 47 Dresden 01307 Germany

抄録

<jats:p>The oligomeric c ring of the F‐ATP synthase from the alkaliphilic cyanobacterium <jats:italic>Spirulina platensis</jats:italic> was isolated and characterized. Mass spectroscopy analysis indicated a mass of 8,210 Da, reflecting that of a c monomer. The mass increased by 206 Da after treatment with the c‐subunit‐specific inhibitor dicyclohexylcarbodiimide (DCCD), which indicated modification of the ion‐binding carboxylate by DCCD. Atomic force microscopy topographs of c rings from <jats:italic>S. platensis</jats:italic> showed 15 symmetrically assembled subunits. The c<jats:sub>15</jats:sub>‐mer reported here is the largest c ring that is isolated and does not show the classical c‐ring mismatch to the three‐fold symmetry of the F<jats:sub>1</jats:sub> domain.</jats:p>

収録刊行物

  • EMBO reports

    EMBO reports 6 (11), 1040-1044, 2005-11

    Springer Science and Business Media LLC

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