Primate Embryonic Stem Cell-Derived Neuronal Progenitors Transplanted into Ischemic Brain

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  • Junya Hayashi
    Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
  • Yasushi Takagi
    Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
  • Hitoshi Fukuda
    Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
  • Takayuki Imazato
    Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
  • Masaki Nishimura
    Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
  • Motoaki Fujimoto
    Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
  • Jun Takahashi
    Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
  • Nobuo Hashimoto
    Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
  • Kazuhiko Nozaki
    Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan

抄録

<jats:p> Transplantation of stem cells has the possibility of restoring neural functions after stroke damage. Therefore, we transplanted neuronal progenitors generated from monkey embryonic stem (ES) cells into the ischemic mouse brain to test this possibility. Monkey ES cells were caused to differentiate into neuronal progenitors by the stromal cell-derived inducing activity method. Focal cerebral ischemia was induced by occluding the middle cerebral artery by the intraluminal filament technique. The donor cells were transplanted into the ischemic lateral striatum at 24 h after the start of reperfusion. The cells transplanted into the ischemic brain became located widely around the ischemic area, and, moreover, the transplanted cells differentiated into various types of neurons and glial cells. Furthermore, at 28 days after the transplantation, over 10 times more cells in the graft were labeled with Fluorogold (FG) by stereotactic focal injection of FG into the anterior thalamus and substantia nigra on the grafted side when compared with the number at 14 days. From these results we confirmed the survival and differentiation of, as well as network formation by, monkey ES-cell-derived neuronal progenitors transplanted into the ischemic mouse brain. </jats:p>

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