A Polycomb group protein complex with sequence-specific DNA-binding and selective methyl-lysine-binding activities
Abstract
<jats:p>Polycomb response elements (PREs) are specific<jats:italic>cis</jats:italic>-regulatory sequences needed for transcriptional repression of HOX and other target genes by Polycomb group (PcG) proteins. Among the many PcG proteins known in<jats:italic>Drosophila</jats:italic>, Pho is the only sequence-specific DNA-binding protein. To gain insight into the function of Pho, we purified Pho protein complexes from<jats:italic>Drosophila</jats:italic>embryos and found that Pho exists in two distinct protein assemblies: a Pho–dINO80 complex containing the<jats:italic>Drosophila</jats:italic>INO80 nucleosome-remodeling complex, and a Pho-repressive complex (PhoRC) containing the uncharacterized gene product dSfmbt. Analysis of PhoRC reveals that dSfmbt is a novel PcG protein that is essential for HOX gene repression in<jats:italic>Drosophila</jats:italic>. PhoRC is bound at HOX gene PREs in vivo, and this targeting strictly depends on Pho-binding sites. Characterization of dSfmbt protein shows that its MBT repeats have unique discriminatory binding activity for methylated lysine residues in histones H3 and H4; the MBT repeats bind mono- and di-methylated H3-K9 and H4-K20 but fail to interact with these residues if they are unmodified or tri-methylated. Our results establish PhoRC as a novel<jats:italic>Drosophila</jats:italic>PcG protein complex that combines DNA-targeting activity (Pho) with a unique modified histone-binding activity (dSfmbt). We propose that PRE-tethered PhoRC selectively interacts with methylated histones in the chromatin flanking PREs to maintain a Polycomb-repressed chromatin state.</jats:p>
Journal
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- Genes & Development
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Genes & Development 20 (9), 1110-1122, 2006-04-17
Cold Spring Harbor Laboratory
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Keywords
Details 詳細情報について
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- CRID
- 1360011145226148480
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- NII Article ID
- 30018918956
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- ISSN
- 15495477
- 08909369
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- Data Source
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- Crossref
- CiNii Articles