Assembly, Verification, and Initial Annotation of the NIA Mouse 7.4K cDNA Clone Set

• Vincent VanBuren

• Yulan Piao

• Dawood B. Dudekula

• Yong Qian

• Mark G. Carter

• Patrick R. Martin

• Carole A. Stagg

• Uwem C. Bassey

• Kazuhiro Aiba

• Toshio Hamatani

• George J. Kargul

• Amber G. Luo

• Janet Kelso

• Winston Hide

• Minoru S.H. Ko

抄録

A set of 7407 cDNA clones (NIA mouse 7.4K) was assembled from >20 cDNA libraries constructed mainly from early mouse embryos, including several stem cell libraries. The clone set was assembled from embryonic and newborn organ libraries consisting of ∼120,000 cDNA clones, which were initially re-arrayed into a set of ∼11,000 unique cDNA clones. A set of tubes was constructed from the racks in this set to prevent contamination and potential mishandling errors in all further re-arrays. Sequences from this set (11K) were analyzed further for quality and clone identity, and high-quality clones with verified identity were re-arrayed into the final set (7.4K). The set is freely available, and a corresponding database was built to provide comprehensive annotation for those clones with known identity or homology, and has been made available through an extensive Web site that includes many link-outs to external databases and analysis servers. [The sequence data from this study have been submitted to GenBank under accession nos. <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="BQ550036" ext-link-type="gen" xlink:type="simple">BQ550036 –<jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" xlink:href="BQ563104" ext-link-type="gen" xlink:type="simple">BQ563104 .]

収録刊行物

• Genome Research

  Genome Research 12 (12), 1999-2003, 2002-11-12

  Cold Spring Harbor Laboratory

キーワード

• Genetics (clinical)
• Genetics

詳細情報

• CRID: 1362544420668866048
• NII論文ID: 30018929613
• DOI: 10.1101/gr.633802
• ISSN: 15495469
• ISSN: 10889051
• Web Site: https://syndication.highwire.org/content/doi/10.1101/gr.633802
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