Transcriptional Down-Regulation by Abscisic Acid of Pathogenesis-Related β-1,3-Glucanase Genes in Tobacco Cell Cultures1
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- Enea Rezzonico
- Friedrich Miescher Institute, P.O. Box 2543, CH-4002 Basel, Switzerland (R.B., E.R., N.F., F.M.)
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- Nathalie Flury
- Friedrich Miescher Institute, P.O. Box 2543, CH-4002 Basel, Switzerland (R.B., E.R., N.F., F.M.)
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- Frederick Meins
- Friedrich Miescher Institute, P.O. Box 2543, CH-4002 Basel, Switzerland (R.B., E.R., N.F., F.M.)
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- Roland Beffa
- Friedrich Miescher Institute, P.O. Box 2543, CH-4002 Basel, Switzerland (R.B., E.R., N.F., F.M.)
抄録
<jats:title>Abstract</jats:title> <jats:p>Class I isoforms of β-1,3-glucanases (βGLU I) and chitinases (CHN I) are antifungal, vacuolar proteins implicated in plant defense. Tobacco (Nicotiana tabacum L.) βGLU I and CHN I usually exhibit tightly coordinated developmental, hormonal, and pathogenesis-related regulation. Both enzymes are induced in cultured cells and tissues of cultivar Havana 425 tobacco by ethylene and are down-regulated by combinations of the growth hormones auxin and cytokinin. We report a novel pattern of βGLU I and CHN I regulation in cultivar Havana 425 tobacco pith-cell suspensions and cultured leaf explants. Abscisic acid (ABA) at a concentration of 10 μmmarkedly inhibited the induction of βGLU I but not of CHN I. RNA-blot hybridization and immunoblot analysis showed that only class I isoforms of βGLU and CHN are induced in cell culture and that ABA inhibits steady-state βGLU I mRNA accumulation. Comparable inhibition of β-glucuronidase expression by ABA was observed for cells transformed with a tobacco βGLU I gene promoter/β-glucuronidase reporter gene fusion. Taken together, the results strongly suggest that ABA down-regulates transcription of βGLU I genes. This raises the possibility that some of the ABA effects on plant-defense responses might involve βGLU I.</jats:p>
収録刊行物
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- Plant Physiology
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Plant Physiology 117 (2), 585-592, 1998-06-01
Oxford University Press (OUP)
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詳細情報 詳細情報について
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- CRID
- 1363951795149513216
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- NII論文ID
- 30019359297
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- ISSN
- 15322548
- 00320889
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