RNA Polymerase Subunits Encoded by the Plastid <i>rpo</i>Genes Are Not Shared with the Nucleus-Encoded Plastid Enzyme1

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  • Germán Serino
    Waksman Institute, Rutgers, The State University of New Jersey, 190 Frulinghuysen Road, Piscataway, New Jersey 08854–8020
  • Pal Maliga
    Waksman Institute, Rutgers, The State University of New Jersey, 190 Frulinghuysen Road, Piscataway, New Jersey 08854–8020

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<jats:title>Abstract</jats:title> <jats:p>Plastid genes in photosynthetic higher plants are transcribed by at least two RNA polymerases. The plastid rpoA, rpoB, rpoC1, and rpoC2 genes encode subunits of the plastid-encoded plastid RNA polymerase (PEP), an Escherichia coli-like core enzyme. The second enzyme is referred to as the nucleus-encoded plastid RNA polymerase (NEP), since its subunits are assumed to be encoded in the nucleus. Promoters for NEP have been previously characterized in tobacco plants lacking PEP due to targeted deletion ofrpoB (encoding the β-subunit) from the plastid genome. To determine if NEP and PEP share any essential subunits, therpoA, rpoC1, and rpoC2genes encoding the PEP α-, β′-, and β"-subunits were removed by targeted gene deletion from the plastid genome. We report here that deletion of each of these genes yielded photosynthetically defective plants that lack PEP activity while maintaining transcription specificity from NEP promoters. Therefore, rpoA,rpoB, rpoC1, and rpoC2 encode PEP subunits that are not essential components of the NEP transcription machinery. Furthermore, our data indicate that no functional copy ofrpoA, rpoB, rpoC1, orrpoC2 that could complement the deleted plastidrpo genes exists outside the plastids.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 117 (4), 1165-1170, 1998-08-01

    Oxford University Press (OUP)

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