Expression of Aluminum-Induced Genes in Transgenic Arabidopsis Plants Can Ameliorate Aluminum Stress and/or Oxidative Stress

DOI PDF 被引用文献18件
  • Bunichi Ezaki
    Research Institute For Bioresources, Okayama University, 2–20–1 Chuou, Kurashiki, Okayama 710–0046, Japan (B.E., H.M.); and
  • Richard C. Gardner
    School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand (R.C.G., Y.E.)
  • Yuka Ezaki
    School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand (R.C.G., Y.E.)
  • Hideaki Matsumoto
    Research Institute For Bioresources, Okayama University, 2–20–1 Chuou, Kurashiki, Okayama 710–0046, Japan (B.E., H.M.); and

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<jats:title>Abstract</jats:title> <jats:p>To examine the biological role of Al-stress-induced genes, nine genes derived from Arabidopsis, tobacco (Nicotiana tabacum L.), wheat (Triticum aestivum L.), and yeast (Saccharomyces cerevisiae) were expressed in Arabidopsis ecotype Landsberg. Lines containing eight of these genes were phenotypically normal and were tested in root elongation assays for their sensitivity to Al, Cd, Cu, Na, Zn, and to oxidative stresses. An Arabidopsis blue-copper-binding protein gene (AtBCB), a tobacco glutathione S-transferase gene (parB), a tobacco peroxidase gene (NtPox), and a tobacco GDP-dissociation inhibitor gene (NtGDI1) conferred a degree of resistance to Al. Two of these genes, AtBCB andparB, and a peroxidase gene from Arabidopsis (AtPox) also showed increased resistance to oxidative stress induced by diamide, while parB conferred resistance to Cu and Na. Al content of Al-treated root tips was reduced in the four Al-resistant plant lines compared with wild-type Ler-0, as judged by morin staining. All four Al-resistant lines also showed reduced staining of roots with 2′,7′-dichloro fluorescein diacetate (H2DCFDA), an indicator of oxidative stress. We conclude that Al-induced genes can serve to protect against Al toxicity, and also provide genetic evidence for a link between Al stress and oxidative stress in plants.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 122 (3), 657-666, 2000-03-01

    Oxford University Press (OUP)

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