A Plastid Terminal Oxidase Associated with Carotenoid Desaturation during Chromoplast Differentiation

DOI PDF 被引用文献5件
  • Eve-Marie Josse
    Laboratoire de Génétique Moléculaire des Plantes, Université Joseph Fourier and Centre National de la Recherche Scientifique (Unité Mixte de Recherche 5575), BP53, 38041 Grenoble cedex 9, France
  • Andrew J. Simkin
    Laboratoire de Génétique Moléculaire des Plantes, Université Joseph Fourier and Centre National de la Recherche Scientifique (Unité Mixte de Recherche 5575), BP53, 38041 Grenoble cedex 9, France
  • Joël Gaffé
    Laboratoire de Génétique Moléculaire des Plantes, Université Joseph Fourier and Centre National de la Recherche Scientifique (Unité Mixte de Recherche 5575), BP53, 38041 Grenoble cedex 9, France
  • Anne-Marie Labouré
    Laboratoire de Génétique Moléculaire des Plantes, Université Joseph Fourier and Centre National de la Recherche Scientifique (Unité Mixte de Recherche 5575), BP53, 38041 Grenoble cedex 9, France
  • Marcel Kuntz
    Laboratoire de Génétique Moléculaire des Plantes, Université Joseph Fourier and Centre National de la Recherche Scientifique (Unité Mixte de Recherche 5575), BP53, 38041 Grenoble cedex 9, France
  • Pierre Carol
    Laboratoire de Génétique Moléculaire des Plantes, Université Joseph Fourier and Centre National de la Recherche Scientifique (Unité Mixte de Recherche 5575), BP53, 38041 Grenoble cedex 9, France

抄録

<jats:title>Abstract</jats:title> <jats:p>The Arabidopsis IMMUTANS gene encodes a plastid homolog of the mitochondrial alternative oxidase, which is associated with phytoene desaturation. Upon expression in Escherichia coli, this protein confers a detectable cyanide-resistant electron transport to isolated membranes. In this assay this activity is sensitive to n-propyl-gallate, an inhibitor of the alternative oxidase. This protein appears to be a plastid terminal oxidase (PTOX) that is functionally equivalent to a quinol:oxygen oxidoreductase. This protein was immunodetected in achlorophyllous pepper (Capsicum annuum) chromoplast membranes, and a corresponding cDNA was cloned from pepper and tomato (Lycopersicum esculentum) fruits. Genomic analysis suggests the presence of a single gene in these organisms, the expression of which parallels phytoene desaturase and ζ-carotene desaturase gene expression during fruit ripening. Furthermore, thisPTOX gene is impaired in the tomato ghostmutant, which accumulates phytoene in leaves and fruits. These data show that PTOX also participates in carotenoid desaturation in chromoplasts in addition to its role during early chloroplast development.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 123 (4), 1427-1436, 2000-08-01

    Oxford University Press (OUP)

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