Greening under High Light or Cold Temperature Affects the Level of Xanthophyll-Cycle Pigments, Early Light-Inducible Proteins, and Light-Harvesting Polypeptides in Wild-Type Barley and the<i>Chlorina f2</i>Mutant1
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- Marianna Król
- Department of Plant Sciences, The University of Western Ontario, London, Ontario, Canada N6A 5B7 (M.K., A.G.I., N.P.A.H.)
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- Alexander G. Ivanov
- Department of Plant Sciences, The University of Western Ontario, London, Ontario, Canada N6A 5B7 (M.K., A.G.I., N.P.A.H.)
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- Stefan Jansson
- Department of Plant Physiology, University of Umeå, S 901 87 Umeå, Sweden (S.J.)
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- Klaus Kloppstech
- Institut für Botanik, Universität Hannover, Herrnhäuser Strasse 2, Hannover 21 3000, Germany (K.K.)
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- Norman P.A. Huner
- Department of Plant Sciences, The University of Western Ontario, London, Ontario, Canada N6A 5B7 (M.K., A.G.I., N.P.A.H.)
抄録
<jats:title>Abstract</jats:title> <jats:p>Etiolated seedlings of wild type and the chlorina f2 mutant of barley (Hordeum vulgare) were exposed to greening at either 5°C or 20°C and continuous illumination varying from 50 to 800 μmol m−2s−1. Exposure to either moderate temperature and high light or low temperature and moderate light inhibited chlorophylla and b accumulation in the wild type and in the f2 mutant. Continuous illumination under these greening conditions resulted in transient accumulations of zeaxanthin, concomitant transient decreases in violaxanthin, and fluctuations in the epoxidation state of the xanthophyll pool. Photoinhibition-induced xanthophyll-cycle activity was detectable after only 3 h of greening at 20°C and 250 μmol m−2 s−1. Immunoblot analyses of the accumulation of the 14-kD early light-inducible protein but not the major (Lhcb2) or minor (Lhcb5) light-harvesting polypeptides demonstrated transient kinetics similar to those observed for zeaxanthin accumulation during greening at either 5°C or 20°C for both the wild type and the f2mutant. Furthermore, greening of the f2 mutant at either 5°C or 20°C indicated that Lhcb2 is not essential for the regulation of the xanthophyll cycle in barley. These results are consistent with the thesis that early light-inducible proteins may bind zeaxanthin as well as other xanthophylls and dissipate excess light energy to protect the developing photosynthetic apparatus from excess excitation. We discuss the role of energy balance and photosystem II excitation pressure in the regulation of the xanthophyll cycle during chloroplast biogenesis in wild-type barley and the f2mutant.</jats:p>
収録刊行物
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- Plant Physiology
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Plant Physiology 120 (1), 193-204, 1999-05-01
Oxford University Press (OUP)
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詳細情報 詳細情報について
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- CRID
- 1360861710878546944
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- NII論文ID
- 30019360473
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- ISSN
- 15322548
- 00320889
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