Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst

DOI Web Site 被引用文献18件
  • Woo Suk Hwang
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Young June Ryu
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Jong Hyuk Park
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Eul Soon Park
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Eu Gene Lee
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Ja Min Koo
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Hyun Yong Jeon
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Byeong Chun Lee
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Sung Keun Kang
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Sun Jong Kim
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Curie Ahn
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Jung Hye Hwang
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Ky Young Park
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Jose B. Cibelli
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
  • Shin Yong Moon
    College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.

抄録

<jats:p>Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells.</jats:p>

収録刊行物

  • Science

    Science 303 (5664), 1669-1674, 2004-03-12

    American Association for the Advancement of Science (AAAS)

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