<i>fgf20</i>
            Is Essential for Initiating Zebrafish Fin Regeneration

Geoffrey G. Whitehead, Shinji Makino, Ching-Ling Lien, Mark T. Keating

doi:10.1126/science.1117637

<i>fgf20</i> Is Essential for Initiating Zebrafish Fin Regeneration

DOI Web Site 被引用文献15件

Geoffrey G. Whitehead

Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Department of Cardiology, Children's Hospital, Boston, MA 02115, USA.
Shinji Makino

Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Department of Cardiology, Children's Hospital, Boston, MA 02115, USA.
Ching-Ling Lien

Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Department of Cardiology, Children's Hospital, Boston, MA 02115, USA.
Mark T. Keating

Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Department of Cardiology, Children's Hospital, Boston, MA 02115, USA.

抄録

<jats:p> Epimorphic regeneration requires the presence or creation of pluripotent cells capable of reproducing lost organs. Zebrafish fin regeneration is mediated by the creation of blastema cells. Here, we characterize the <jats:italic>devoid of blastema</jats:italic> ( <jats:italic>dob</jats:italic> ) mutant that fails fin regeneration during initial steps, forms abnormal regeneration epithelium, and does not form blastema. This mutation has no impact on embryonic survival. <jats:italic>Dob</jats:italic> results from an <jats:italic>fgf20a</jats:italic> null mutation, Y148S. <jats:italic>Fgf20a</jats:italic> is expressed during initiation of fin regeneration at the epithelial-mesenchymal boundary and later overlaps with the blastema marker <jats:italic>msxb</jats:italic> . Thus, <jats:italic>fgf20a</jats:italic> has a regeneration-specific requirement, initiating fin regeneration, and controlling blastema formation. </jats:p>