Nucleotide Sequence and Expression of an AIDS-Associated Retrovirus (ARV-2)

  • Ray Sanchez-Pescador
    Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608,
  • Michael D. Power
    Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608,
  • Philip J. Barr
    Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608,
  • Kathelyn S. Steimer
    Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608,
  • Michelle M. Stempien
    Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608,
  • Sheryl L. Brown-Shimer
    Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608,
  • Wendy W. Gee
    Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608,
  • Andre Renard
    Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608,
  • Anne Randolph
    Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608,
  • Jay A. Levy
    Cancer Research Institute, University of California, School of Medicine, San Francisco, California 94143.
  • Dino Dina
    Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608,
  • Paul A. Luciw
    Chiron Research Laboratories, Chiron Corporation, Emeryville, California 94608,

抄録

<jats:p> The nucleotide sequence of molecular clones of DNA from a retrovirus, ARV-2, associated with the acquired immune deficiency syndrome (AIDS) was determined. Proviral DNA of ARV-2 (9737 base pairs) has long terminal repeat structures (636 base pairs) and long open reading frames encoding <jats:italic>gag</jats:italic> (506 codons), <jats:italic>pol</jats:italic> (1003 codons), and <jats:italic>env</jats:italic> (863 codons) genes. Two additional open reading frames were identified. Significant amino acid homology with several other retroviruses was noted in the predicted product of <jats:italic>gag</jats:italic> and <jats:italic>pol</jats:italic> , but ARV-2 was as closely related to murine and avian retroviruses as it was to human T-cell leukemia viruses (HTLV-I and HTLV-II). By means of an SV-40 vector in transfected simian cells, the cloned <jats:italic>gag</jats:italic> and <jats:italic>env</jats:italic> genes of ARV-2 were shown to express viral proteins. </jats:p>

収録刊行物

  • Science

    Science 227 (4686), 484-492, 1985-02

    American Association for the Advancement of Science (AAAS)

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