Hypomethylation of DNA from Benign and Malignant Human Colon Neoplasms

DOI Web Site 6 Citations
  • Susan E. Goelz
    Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
  • Bert Vogelstein
    Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205
  • Stanley R. Hamilton
    Department of Pathology, Johns Hopkins University School of Medicine
  • Andrew P. Feinberg
    Oncology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Abstract

<jats:p>The methylation state of DNA from human colon tissue displaying neoplastic growth was determined by means of restriction endonuclease analysis. When compared to DNA from adjacent normal tissue, DNA from both benign colon polyps and malignant carcinomas was substantially hypomethylated. With the use of probes for growth hormone, γ-globin, α-chorionic gonadotropin, and γ-crystallin, methylation changes were detected in all 23 neoplastic growths examined. Benign polyps were hypomethylated to a degree similar to that in malignant tissue. These results indicate that hypomethylation is a consistent biochemical characteristic of human colonic tumors and is an alteration in the DNA that precedes malignancy.</jats:p>

Journal

  • Science

    Science 228 (4696), 187-190, 1985-04-12

    American Association for the Advancement of Science (AAAS)

Citations (6)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top