Molecular and Biochemical Heterogeneity of Class B Carbapenem-Hydrolyzing β-Lactamases in <i>Chryseobacterium meningosepticum</i>
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- Samuel Bellais
- <!--label omitted: 1-->Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre Cedex, France
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- Daniel Aubert
- <!--label omitted: 1-->Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre Cedex, France
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- Thierry Naas
- <!--label omitted: 1-->Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre Cedex, France
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- Patrice Nordmann
- <!--label omitted: 1-->Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, 94275 Le Kremlin-Bicêtre Cedex, France
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<jats:title>ABSTRACT</jats:title> <jats:p> Although the carbapenem-hydrolyzing β-lactamase (CHβL) BlaB-1 is known to be in <jats:italic>Chryseobacterium meningosepticum</jats:italic> NCTC 10585, a second CHβL gene, <jats:italic>bla</jats:italic> <jats:sub>GOB-1</jats:sub> , was cloned from another <jats:italic>C. meningosepticum</jats:italic> clinical isolate (PINT). The G+C content of <jats:italic>bla</jats:italic> <jats:sub>GOB-1</jats:sub> (36%) indicated the likely chromosomal origin of this gene. Its expression in <jats:italic>Escherichia coli</jats:italic> DH10B yields a mature CHβL with a pI of 8.7 and a relative molecular mass of 28.2 kDa. In <jats:italic>E. coli</jats:italic> , GOB-1 conferred resistance to narrow-spectrum cephalosporins and reduced susceptibility to ureidopenicillins, broad-spectrum cephalosporins, and carbapenems. GOB-1 had a broad-spectrum hydrolysis profile including penicillins and cephalosporins (but not aztreonam). The catalytic efficiency for meropenem was higher than for imipenem. GOB-1 had low amino acid identity with the class B CHβLs, sharing 18% with the closest, L-1 from <jats:italic>Stenotrophomonas maltophilia</jats:italic> , and only 11% with BlaB-1. Most of the conserved amino acids that may be involved in the active site of CHβLs (His-101, Asp-103, His-162, and His-225) were identified in GOB-1. Sequence heterogeneity was found for GOB-1-like and BlaB-1-like β-lactamases, having 90 to 100% and 86 to 100% amino acid identity, respectively, among 10 unrelated <jats:italic>C. meningosepticum</jats:italic> isolates. Each isolate had a GOB-1-like and a BlaB-1-like gene. The same combination of GOB-1-like and BlaB-1-like β-lactamases was not found in two different isolates. <jats:italic>C. meningosepticum</jats:italic> is a bacterial species with two types of unrelated chromosome-borne class B CHβLs that can be expressed in <jats:italic>E. coli</jats:italic> and, thus, may represent a clinical threat if spread in gram-negative aerobes. </jats:p>
収録刊行物
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- Antimicrobial Agents and Chemotherapy
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Antimicrobial Agents and Chemotherapy 44 (7), 1878-1886, 2000-07
American Society for Microbiology
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詳細情報 詳細情報について
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- CRID
- 1361137046025333504
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- NII論文ID
- 30020629494
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- NII書誌ID
- AA00542574
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- ISSN
- 10986596
- 00664804
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