Lysostaphin Disrupts <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i> Biofilms on Artificial Surfaces

• Julie A. Wu

  Biosynexus Incorporated, Gaithersburg, Maryland

• Caroline Kusuma

  Biosynexus Incorporated, Gaithersburg, Maryland

• James J. Mond

  Biosynexus Incorporated, Gaithersburg, Maryland

• John F. Kokai-Kun

  Biosynexus Incorporated, Gaithersburg, Maryland

抄録

Staphylococci often form biofilms, sessile communities of microcolonies encased in an extracellular matrix that adhere to biomedical implants or damaged tissue. Infections associated with biofilms are difficult to treat, and it is estimated that sessile bacteria in biofilms are 1,000 to 1,500 times more resistant to antibiotics than their planktonic counterparts. This antibiotic resistance of biofilms often leads to the failure of conventional antibiotic therapy and necessitates the removal of infected devices. Lysostaphin is a glycylglycine endopeptidase which specifically cleaves the pentaglycine cross bridges found in the staphylococcal peptidoglycan. Lysostaphin kills Staphylococcus aureus within minutes (MIC at which 90% of the strains are inhibited [MIC 90 ], 0.001 to 0.064 μg/ml) and is also effective against Staphylococcus epidermidis at higher concentrations (MIC 90 , 12.5 to 64 μg/ml). The activity of lysostaphin against staphylococci present in biofilms compared to those of other antibiotics was, however, never explored. Surprisingly, lysostaphin not only killed S. aureus in biofilms but also disrupted the extracellular matrix of S. aureus biofilms in vitro on plastic and glass surfaces at concentrations as low as 1 μg/ml. Scanning electron microscopy confirmed that lysostaphin eradicated both the sessile cells and the extracellular matrix of the biofilm. This disruption of S. aureus biofilms was specific for lysostaphin-sensitive S. aureus , as biofilms of lysostaphin-resistant S. aureus were not affected. High concentrations of oxacillin (400 μg/ml), vancomycin (800 μg/ml), and clindamycin (800 μg/ml) had no effect on the established S. aureus biofilms in this system, even after 24 h. Higher concentrations of lysostaphin also disrupted S. epidermidis biofilms.

収録刊行物

• Antimicrobial Agents and Chemotherapy

  Antimicrobial Agents and Chemotherapy 47 (11), 3407-3414, 2003-11

  American Society for Microbiology

キーワード

• Infectious Diseases
• Pharmacology (medical)
• Pharmacology

詳細情報

• CRID: 1360855569839577600
• NII論文ID: 30020631679
• DOI: 10.1128/aac.47.11.3407-3414.2003
• ISSN: 10986596
• ISSN: 00664804
• Web Site: https://journals.asm.org/doi/pdf/10.1128/AAC.47.11.3407-3414.2003
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