Activation of Natural Killer T Cells by α-Galactosylceramide Impairs DNA Vaccine-Induced Protective Immunity against<i>Trypanosoma cruzi</i>

DOI Web Site 被引用文献2件
  • Yasushi Miyahira
    Department of Molecular and Cellular Parasitology
  • Masaharu Katae
    Department of Molecular and Cellular Parasitology
  • Kazuyoshi Takeda
    Department of Immunology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo 113-8421
  • Hideo Yagita
    Department of Immunology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo 113-8421
  • Ko Okumura
    Department of Immunology, Juntendo University School of Medicine, Bunkyo-ku, Tokyo 113-8421
  • Seiki Kobayashi
    Department of Tropical Medicine and Parasitology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582
  • Tsutomu Takeuchi
    Department of Tropical Medicine and Parasitology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582
  • Tsuneo Kamiyama
    Department of Veterinary Sciences, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan
  • Yoshinosuke Fukuchi
    Department of Respiratory Medicine
  • Takashi Aoki
    Department of Molecular and Cellular Parasitology

この論文をさがす

抄録

<jats:title>ABSTRACT</jats:title><jats:p>Innate immunity as a first defense is indispensable for host survival against infectious agents. We examined the roles of natural killer (NK) T cells in defense against<jats:italic>Trypanosoma cruzi</jats:italic>infection. The<jats:italic>T. cruzi</jats:italic>parasitemia and survival of CD1d-deficient mice exhibited no differences compared to wild-type littermates. NK T-cell activation induced by administering α-galactosylceramide (α-GalCer) to<jats:italic>T. cruzi</jats:italic>-infected mice significantly changed the parasitemia only in the late phase of infection and slightly improved survival when mice were infected intraperitoneally. The combined usage of α-GalCer and benznidazole, a commercially available drug for Chagas' disease, did not enhance the therapeutic efficacy of benznidazole. These results suggest that NK T cells do not play a pivotal role in resistance to<jats:italic>T. cruzi</jats:italic>infection. In addition, we found that the coadministration of α-GalCer with DNA vaccine impaired the induction of epitope-specific CD8<jats:sup>+</jats:sup>T cells and undermined the DNA vaccine-induced protective immunity against<jats:italic>T. cruzi</jats:italic>. Our results, in contrast to previous reports demonstrating the protective roles of NK T cells against other infectious agents, suggest that these cells might even exhibit adverse effects on vaccine-mediated protective immunity.</jats:p>

収録刊行物

被引用文献 (2)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ