Cross-Regulation of Biphenyl- and Salicylate-Catabolic Genes by Two Regulatory Systems in <i>Pseudomonas pseudoalcaligenes</i> KF707

DOI Web Site 2 Citations
  • Hidehiko Fujihara
    Laboratory of Applied Microbiology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan
  • Hideyuki Yoshida
    Laboratory of Applied Microbiology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan
  • Tetsuya Matsunaga
    Laboratory of Applied Microbiology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan
  • Masatoshi Goto
    Laboratory of Applied Microbiology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan
  • Kensuke Furukawa
    Laboratory of Applied Microbiology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan

Abstract

<jats:title>ABSTRACT</jats:title> <jats:p> <jats:italic>Pseudomonas pseudoalcaligenes</jats:italic> KF707 grows on biphenyl and salicylate as sole sources of carbon. The biphenyl-catabolic ( <jats:italic>bph</jats:italic> ) genes are organized as <jats:italic>bphR1A1A2</jats:italic> ( <jats:italic>orf3</jats:italic> ) <jats:italic>A3A4BCX0X1X2X3D</jats:italic> , encoding the enzymes for conversion of biphenyl to acetyl coenzyme A. In this study, the salicylate-catabolic ( <jats:italic>sal</jats:italic> ) gene cluster encoding the enzymes for conversion of salicylate to acetyl coenzyme A were identified 6.6-kb downstream of the <jats:italic>bph</jats:italic> gene cluster along with a second regulatory gene, <jats:italic>bphR2</jats:italic> . Both the <jats:italic>bph</jats:italic> and <jats:italic>sal</jats:italic> genes were cross-regulated positively and/or negatively by the two regulatory proteins, BphR1 and BphR2, in the presence or absence of the effectors. The BphR2 binding sequence exhibits homology with the NahR binding sequences in various naphthalene-degrading bacteria. Based on previous studies and the present study we propose a new regulatory model for biphenyl and salicylate catabolism in strain KF707. </jats:p>

Journal

Citations (2)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top