Identification and Characterization of Genes Involved in the Downstream Degradation Pathway of γ-Hexachlorocyclohexane in <i>Sphingomonas paucimobilis</i> UT26

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  • Ryo Endo
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai
  • Mayuko Kamakura
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai
  • Keisuke Miyauchi
    Department of Bioengineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata, Japan
  • Masao Fukuda
    Department of Bioengineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata, Japan
  • Yoshiyuki Ohtsubo
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai
  • Masataka Tsuda
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai
  • Yuji Nagata
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai

Abstract

<jats:title>ABSTRACT</jats:title> <jats:p> <jats:italic>Sphingomonas paucimobilis</jats:italic> UT26 utilizes γ-hexachlorocyclohexane (γ-HCH) as a sole source of carbon and energy. In our previous study, we cloned and characterized genes that are involved in the conversion of γ-HCH to maleylacetate (MA) via chlorohydroquinone (CHQ) in UT26. In this study, we identified and characterized an MA reductase gene, designated <jats:italic>linF</jats:italic> , that is essential for the utilization of γ-HCH in UT26. A gene named <jats:italic>linEb</jats:italic> , whose deduced product showed significant identity to LinE (53%), was located close to <jats:italic>linF</jats:italic> . LinE is a novel type of ring cleavage dioxygenase that catalyzes the conversion of CHQ to MA. LinEb expressed in <jats:italic>Escherichia coli</jats:italic> transformed CHQ and 2,6-dichlorohydroquinone to MA and 2-chloromaleylacetate, respectively. Our previous and present results indicate that UT26 (i) has two gene clusters for degradation of chlorinated aromatic compounds via hydroquinone-type intermediates and (ii) uses at least parts of both clusters for γ-HCH utilization. </jats:p>

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