Molecular Analysis of Bacterial Species Associated with Childhood Caries

• Mitzi R. Becker

  Department of Pediatric Dentistry

• Bruce J. Paster

  Department of Molecular Genetics, The Forsyth Institute

• Eugene J. Leys

  Department of Oral Biology, College of Dentistry

• Melvin L. Moeschberger

  Division of Epidemiology and Biometrics, School of Public Health, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio

• Sarah G. Kenyon

  Department of Molecular Genetics, The Forsyth Institute

• Jamie L. Galvin

  Department of Molecular Genetics, The Forsyth Institute

• Susan K. Boches

  Department of Molecular Genetics, The Forsyth Institute

• Floyd E. Dewhirst

  Department of Molecular Genetics, The Forsyth Institute

• Ann L. Griffen

  Department of Pediatric Dentistry

抄録

Although substantial epidemiologic evidence links Streptococcus mutans to caries, the pathobiology of caries may involve more complex communities of bacterial species. Molecular methods for bacterial identification and enumeration now make it possible to more precisely study the microbiota associated with dental caries. The purpose of this study was to compare the bacteria found in early childhood caries (ECC) to those found in caries-free children by using molecular identification methods. Cloning and sequencing of bacterial 16S ribosomal DNAs from a healthy subject and a subject with ECC were used for identification of novel species or uncultivated phylotypes and species not previously associated with dental caries. Ten novel phylotypes were identified. A number of species or phylotypes that may play a role in health or disease were identified and warrant further investigation. In addition, quantitative measurements for 23 previously known bacterial species or species groups were obtained by a reverse capture checkerboard assay for 30 subjects with caries and 30 healthy controls. Significant differences were observed for nine species: S . sanguinis was associated with health and, in order of decreasing cell numbers, Actinomyces gerencseriae , Bifidobacterium , S . mutans , Veillonella , S . salivarius , S . constellatus , S . parasanguinis , and Lactobacillus fermentum were associated with caries. These data suggest that A . gerencseriae and other Actinomyces species may play an important role in caries initiation and that a novel Bifidobacterium may be a major pathogen in deep caries. Further investigation could lead to the identification of targets for biological interventions in the caries process and thereby contribute to improved prevention of and treatment for this significant public health problem.

収録刊行物

• Journal of Clinical Microbiology

  Journal of Clinical Microbiology 40 (3), 1001-1009, 2002-03

  American Society for Microbiology

キーワード

• Microbiology (medical)

詳細情報

• CRID: 1363388844725657344
• NII論文ID: 30021102121
• DOI: 10.1128/jcm.40.3.1001-1009.2002
• ISSN: 1098660X
• ISSN: 00951137
• Web Site: https://journals.asm.org/doi/pdf/10.1128/JCM.40.3.1001-1009.2002
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