PCR Typing of Genetic Determinants for Metallo-β-Lactamases and Integrases Carried by Gram-Negative Bacteria Isolated in Japan, with Focus on the Class 3 Integron

• Naohiro Shibata

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan

• Yohei Doi

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan

• Kunikazu Yamane

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan

• Tetsuya Yagi

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan

• Hiroshi Kurokawa

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan

• Keigo Shibayama

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan

• Haru Kato

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan

• Kumiko Kai

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan

• Yoshichika Arakawa

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan

抄録

From January 2001 to December 2002, 587 strains of gram-negative bacterial isolates demonstrating resistance to ceftazidime and a combination of sulbactam and cefoperazone were subjected to a disk diffusion screening test using sodium mercaptoacetic acid; 431 strains (73.4%) appeared to produce metallo-β-lactamase (MBL). Of these 431 strains, 357 were found by PCR to carry genes for IMP-1 type MBL ( bla IMP-1 ), while only 7 and 67 strains carried the IMP-2 gene ( bla IMP-2 ) and the VIM-2 gene ( bla VIM-2 ), respectively. Neither VIM-1 nor SPM-1 type MBL genes were found among the strains tested. Of 431 strains, 427 carried the intI1 gene, and 4 strains carrying both the intI1 and intI3 genes were reidentified as Pseudomonas putida harboring bla IMP-1 . Of these four P. putida strains, three strains and one strain, respectively, were separately isolated from two hospitals located in the same prefecture, and the three strains showed very similar pulsed-field gel electrophoresis patterns. Of 357 bla IMP-1 carriers, 116, 53, 51, 47, and 30 strains were identified as Pseudomonas aeruginosa , Alcaligenes xylosoxidans , P. putida/fluorescens , Serratia marcescens , and Acinetobacter baumannii , respectively. Four strains carrying bla IMP-2 were reidentified as P. putida . Sixty-three P. aeruginosa strains and four P. putida strains carried bla VIM-2 . Of 427 intI1 -positive strains, 180, 53, 51, 47, and 35 were identified as P. aeruginosa , A. xylosoxidans , P. putida/fluorescens , S. marcescens , and A. baumannii , respectively. In the present study, it was confirmed that strains carrying bla IMP-1 with a class 1 integron are the most prevalent type in Japan, although several intI3 carriers have also been identified sporadically in this country.

収録刊行物

• Journal of Clinical Microbiology

  Journal of Clinical Microbiology 41 (12), 5407-5413, 2003-12

  American Society for Microbiology

キーワード

• Microbiology (medical)

詳細情報

• CRID: 1363388844979081856
• NII論文ID: 30021104761
• DOI: 10.1128/jcm.41.12.5407-5413.2003
• ISSN: 1098660X
• ISSN: 00951137
• Web Site: https://journals.asm.org/doi/pdf/10.1128/JCM.41.12.5407-5413.2003
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