Characterization of<i>Salmonella enterica</i>Serotype Newport Isolated from Humans and Food Animals

• S. Zhao

  Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland 20708

• S. Qaiyumi

  Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland 20708

• S. Friedman

  Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland 20708

• R. Singh

  Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland 20708

• S. L. Foley

  Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland 20708

• D. G. White

  Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland 20708

• P. F. McDermott

  Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland 20708

• T. Donkar

  Division of Microbiological Studies, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, Maryland 20740

• C. Bolin

  Animal Health Diagnostic Laboratory, Michigan State University, East Lansing, Michigan 48824

• S. Munro

  Stanford University Medical Center, Stanford, California 94305

• E. J. Baron

  Stanford University Medical Center, Stanford, California 94305

• R. D. Walker

  Division of Animal and Food Microbiology, Office of Research, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland 20708

抄録

Salmonella enterica serotype Newport isolates resistant to at least nine antimicrobials (including extended-spectrum cephalosporins), known as serotype Newport MDR-AmpC isolates, have been rapidly emerging as pathogens in both animals and humans throughout the United States. Resistance to extended-spectrum cephalosporins is associated with clinical failures, including death, in patients with systemic infections. In this study, 87 Salmonella serotype Newport strains were characterized by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility testing and examined for the presence of class 1 integrons and bla CMY genes. Thirty-five PFGE patterns were observed with Xba I, and three of these patterns were indistinguishable among isolates from humans and animals. Fifty-three (60%) Salmonella serotype Newport isolates were identified as serotype Newport MDR-AmpC, including 16 (53%) of 30 human isolates, 27 (93%) of 29 cattle isolates, 7 (70%) of 10 swine isolates, and 3 (30%) of 10 chicken isolates. However, 28 (32%) Salmonella serotype Newport isolates were susceptible to all 16 antimicrobials tested. The bla CMY gene was present in all serotype Newport MDR-AmpC isolates. Furthermore, the plasmid-mediated bla CMY gene was transferable via conjugation to an Escherichia coli strain. The transconjugant showed the MDR-AmpC resistance profile. Thirty-five (40%) of the isolates possessed class 1 integrons. Sequence analyses of the integrons showed that they contained aadA , which confers resistance to streptomycin, or aadA and dhfr , which confer resistance to trimethoprim-sulfamethoxazole. One integron from a swine isolate contained the sat - 1 gene, which encodes resistance to streptothricin, an antimicrobial agent that has never been approved for use in the United States. In conclusion, Salmonella serotype Newport MDR-AmpC was commonly identified among Salmonella serotype Newport isolates recovered from humans and food animals. These findings support the possibility of transmission of this organism to humans through the food chain.

収録刊行物

• Journal of Clinical Microbiology

  Journal of Clinical Microbiology 41 (12), 5366-5371, 2003-12

  American Society for Microbiology

キーワード

• Microbiology (medical)

詳細情報

• CRID: 1363107369851794304
• NII論文ID: 30021104780
• DOI: 10.1128/jcm.41.12.5366-5371.2003
• ISSN: 1098660X
• ISSN: 00951137
• Web Site: https://journals.asm.org/doi/pdf/10.1128/JCM.41.12.5366-5371.2003
• データソース種別:
  • Crossref
  • CiNii Articles