Detection of Human Metapneumovirus Antigens in Nasopharyngeal Secretions by an Immunofluorescent-Antibody Test

DOI Web Site 被引用文献7件
  • Takashi Ebihara
    Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Rika Endo
    Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Xiaoming Ma
    Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Nobuhisa Ishiguro
    Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Japan
  • Hideaki Kikuta
    Department of Pediatrics, Hokkaido University Graduate School of Medicine, Sapporo, Japan

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<jats:title>ABSTRACT</jats:title> <jats:p>Human metapneumovirus (hMPV) is a recently discovered pathogen associated with respiratory tract infections, primarily in young children, immunocompromised individuals, and elderly individuals. Reverse transcription-PCR (RT-PCR) has been reported to be a more sensitive method for the diagnosis of hMPV infections than virus isolation by culture and serological study. However, there has been no report on rapid methods, such as an immunofluorescent-antibody test or an enzyme-linked immunosorbent assay, for the detection of hMPV antigens in nasopharyngeal secretions. In this study, we compared an indirect immunofluorescent-antibody test (IFA) with a monoclonal antibody with RT-PCR for detection of hMPV in nasal secretions from 48 hospitalized children with respiratory tract infections. Fifteen of the 48 children were positive for hMPV by RT-PCR. IFA results were positive for 11 of the 15 RT-PCR-positive children (sensitivity, 73.3%) and 1 of the 33 RT-PCR-negative children (specificity, 97.0%). Although the sensitivity of IFA is lower than that of RT-PCR, IFA is a rapid and useful test for the diagnosis of hMPV infections in children.</jats:p>

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