Hedgehog Proteins Stimulate Chondrogenic Cell Differentiation and Cartilage Formation
-
- Motomi Enomoto-Iwamoto
- Department of Biochemistry, Osaka University Faculty of Dentistry, Osaka, Japan
-
- Takashi Nakamura
- The First Department of Oral and Maxillofacial Surgery, Osaka University Faculty of Dentistry, Osaka, Japan
-
- Tomonao Aikawa
- The First Department of Oral and Maxillofacial Surgery, Osaka University Faculty of Dentistry, Osaka, Japan
-
- Yoshinobu Higuchi
- Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, Osaka, Japan
-
- Takahito Yuasa
- Department of Oral Pathology, School of Dentistry, Nagasaki University, Nagasaki, Japan
-
- Akira Yamaguchi
- Department of Oral Pathology, School of Dentistry, Nagasaki University, Nagasaki, Japan
-
- Tsutomu Nohno
- Department of Molecular Biology, Kawasaki Medical School, Kurashiki, Japan
-
- Sumihare Noji
- Department of Biological Science and Technology, Faculty of Engineering, University of Tokushima, Tokushima, Japan
-
- Tokuzo Matsuya
- The First Department of Oral and Maxillofacial Surgery, Osaka University Faculty of Dentistry, Osaka, Japan
-
- Kojiro Kurisu
- Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, Osaka, Japan
-
- Eiki Koyama
- Department of Anatomy and Histology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, U.S.A.
-
- Maurizio Pacifici
- Department of Anatomy and Histology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, U.S.A.
-
- Masahiro Dr. Iwamoto
- Department of Oral Anatomy and Developmental Biology, Osaka University Faculty of Dentistry, Osaka, Japan
Abstract
<jats:title>Abstract</jats:title> <jats:p>Sonic hedgehog (Shh) and Indian hedgehog (Ihh) are important regulators of skeletogenesis, but their roles in this complex multistep process are not fully understood. Recent studies have suggested that the proteins participate in the differentiation of chondrogenic precursor cells into chondrocytes. In the present study, we have tested this possibility more directly. We found that implantation of dermal fibroblasts expressing hedgehog proteins into nude mice induces ectopic cartilage and bone formation. Immunohistological and reverse-transcription polymerase chain reaction (RT-PCR) analyses revealed that the ectopic tissues derived largely if not exclusively from host cells. We found also that treatment of clonal prechondrogenic RMD-1 and ATDC5 cells in culture with Ihh or recombinant amino half of Shh (recombinant N-terminal portion of Shh [rShh-N]) induced their differentiation into chondrocytes, as revealed by cytoarchitectural changes, Alcian blue staining and proteoglycan synthesis. Induction of RMD-1 cell differentiation by Ihh or rShh-N was synergistically enhanced by cotreatment with bone morphogenetic protein 2 (BMP-2) but was blocked by cotreatment with fibroblast growth factor 2 (FGF-2). Our findings indicate that hedgehog proteins have the ability to promote differentiation of chondrogenic precursor cells and that their action in this process can be influenced and modified by synergistic or antagonist cofactors.</jats:p>
Journal
-
- Journal of Bone and Mineral Research
-
Journal of Bone and Mineral Research 15 (9), 1659-1668, 2000-09-01
Oxford University Press (OUP)
- Tweet
Details 詳細情報について
-
- CRID
- 1361699994335648896
-
- NII Article ID
- 30021656976
-
- ISSN
- 15234681
- 08840431
-
- Web Site
- https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1359%2Fjbmr.2000.15.9.1659
- https://onlinelibrary.wiley.com/doi/pdf/10.1359/jbmr.2000.15.9.1659
- https://onlinelibrary.wiley.com/doi/full-xml/10.1359/jbmr.2000.15.9.1659
- https://academic.oup.com/jbmr/article-pdf/15/9/1659/56603032/5650150903.pdf
-
- Data Source
-
- Crossref
- CiNii Articles