Fine Surface lmages That Reflect Cytoskeletal Structures in Cultured Glid Cells by Atomic Force Microscopy

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  • Yamane Yukako
    Divisions of Biological Sciences. Graduate School of Science, Hokkaido University, Kita–ku, Sapporo 060, Japan
  • Hatakeyama Dai
    Divisions of Biological Sciences. Graduate School of Science, Hokkaido University, Kita–ku, Sapporo 060, Japan
  • Tojima Takuro
    Divisions of Biological Sciences. Graduate School of Science, Hokkaido University, Kita–ku, Sapporo 060, Japan
  • Kawabata Kazushige
    Divisions of Biological Physics, Graduate School of Science, Hokkaido University, Kita–ku, Sapporo 060, Japan
  • Ushiki Tatsuo
    Department of Anatomy and Histology, Niigata University School of Medicine, Asahimachi–dori, Niigata 951, Japan
  • Ogura Shigeaki
    Departments of Medicine. Hokkaido University School of Medicine, Kita–ku, Sapporo 060, Japan
  • Abe Kazuhiro
    Departments of Anatomy, Hokkaido University School of Medicine, Kita–ku, Sapporo 060, Japan
  • Ito Etsuro
    Divisions of Biological Sciences. Graduate School of Science, Hokkaido University, Kita–ku, Sapporo 060, Japan

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タイトル別名
  • Fine Surface Images That Reflect Cytoskeletal Structures in Cultured Glial Cells by Atomic Force Microscopy.
  • Fine Surface lmages That Reflect Cytosk

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The morphology of cultured glial cells was examined using a combination of atomic force microscopy (AFM) and immunofluorescence staining for cytoskeletons. The meshwork of type-1 astrocytes consisted of thick longitudinal and thin lateral lines on the cell surfaces observed by AFM; the former lines were confirmed to be reflections of actin filaments. The astrocytic processes of type-2 astrocytes were observed to be rugged on AFM. These structures were mainly affected by microtubules. Immunofluorescence imaging of microglia revealed that actin filaments and microtubules were arranged radially and wavily along the cell edge, respectively. AFM could detect these radial and wavy structures clearly. These results show that AFM can provide information on the cytoskeletons of glial cells, indicating that AFM is a useful tool for the morphological characterization of cells.

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