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- R. G. Birch
- Department of Botany, The University of Queensland, Brisbane, 4072, Australia
抄録
<jats:p> ▪ Abstract Plant transformation is now a core research tool in plant biology and a practical tool for cultivar improvement. There are verified methods for stable introduction of novel genes into the nuclear genomes of over 120 diverse plant species. This review examines the criteria to verify plant transformation; the biological and practical requirements for transformation systems; the integration of tissue culture, gene transfer, selection, and transgene expression strategies to achieve transformation in recalcitrant species; and other constraints to plant transformation including regulatory environment, public perceptions, intellectual property, and economics. Because the costs of screening populations showing diverse genetic changes can far exceed the costs of transformation, it is important to distinguish absolute and useful transformation efficiencies. The major technical challenge facing plant transformation biology is the development of methods and constructs to produce a high proportion of plants showing predictable transgene expression without collateral genetic damage. This will require answers to a series of biological and technical questions, some of which are defined. </jats:p>
収録刊行物
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- Annual Review of Plant Physiology and Plant Molecular Biology
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Annual Review of Plant Physiology and Plant Molecular Biology 48 (1), 297-326, 1997-06
Annual Reviews
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詳細情報 詳細情報について
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- CRID
- 1364233270376944000
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- NII論文ID
- 30022123185
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- ISSN
- 10402519
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- データソース種別
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