Temperature‐Dependency of Rigor‐Mortis of Fish Muscle: Myofibrillar Mg<sup>2+</sup>‐ATPase Activity and Ca<sup>2+</sup> Uptake by Sarcoplasmic Reticulum

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<jats:title>ABSTRACT</jats:title><jats:p>Myofibrillar Mg<jats:sup>2+</jats:sup>‐ATPase activity of plaice and red sea bream increased with increase in reaction temperature and Ca<jats:sup>2+</jats:sup> concentration. However, temperature decrease tended to lower Ca<jats:sup>2+</jats:sup> sensitivity of Mg<jats:sup>2+</jats:sup>‐ATPase activity. While it was assumed that spiked fish muscle was relaxed and the myofibrillar Ca<jats:sup>2+</jats:sup> concentration was low, the progress rate of rigor‐mortis correlated well with Mg<jats:sup>2+</jats:sup>‐ATPase activity in the presence of a few micromolar Ca<jats:sup>2+</jats:sup> at 0°C. Correspondingly, it was found that Ca<jats:sup>2+</jats:sup> uptake by plaice sarcoplasmic reticulum decreased with the decrease in reaction temperature. These results strongly suggest the acceleration of fish rigor‐mortis at 0°C is due to the increase of Ca<jats:sup>2+</jats:sup> concentration in myofibrils during storage at this temperature.</jats:p>

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