Adenosine affects expression of membrane molecules, cytokine and chemokine release, and the T-cell stimulatory capacity of human dendritic cells

DOI PDF 被引用文献4件
  • Elisabeth Panther
    From the Department of Experimental Dermatology and Pneumology, University of Freiburg, Germany; and the Laboratory of Immunology, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Roma, Italy
  • Silvia Corinti
    From the Department of Experimental Dermatology and Pneumology, University of Freiburg, Germany; and the Laboratory of Immunology, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Roma, Italy
  • Marco Idzko
    From the Department of Experimental Dermatology and Pneumology, University of Freiburg, Germany; and the Laboratory of Immunology, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Roma, Italy
  • Yared Herouy
    From the Department of Experimental Dermatology and Pneumology, University of Freiburg, Germany; and the Laboratory of Immunology, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Roma, Italy
  • Matthias Napp
    From the Department of Experimental Dermatology and Pneumology, University of Freiburg, Germany; and the Laboratory of Immunology, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Roma, Italy
  • Andrea la Sala
    From the Department of Experimental Dermatology and Pneumology, University of Freiburg, Germany; and the Laboratory of Immunology, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Roma, Italy
  • Giampiero Girolomoni
    From the Department of Experimental Dermatology and Pneumology, University of Freiburg, Germany; and the Laboratory of Immunology, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Roma, Italy
  • Johannes Norgauer
    From the Department of Experimental Dermatology and Pneumology, University of Freiburg, Germany; and the Laboratory of Immunology, Istituto Dermopatico dell'Immacolata, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Roma, Italy

抄録

<jats:title>Abstract</jats:title><jats:p>Dendritic cells (DCs) express functional purinergic type 1 receptors, but the effects of adenosine in these antigen-presenting cells have been only marginally investigated. Here, we further characterized the biologic activity of adenosine in immature DCs (iDCs) and lipopolysaccharide (LPS)–matured DCs (mDCs). Chronic stimulation with adenosine enhanced the macropinocytotic activity and the membrane expression of CD80, CD86, major histocompatibility complex (MHC) class I, and HLA-DR molecules on iDCs. Adenosine also increased LPS-induced CD54, CD80, MHC class I, and HLA-DR molecule expression in mDCs. In addition, adenosine dose-dependently inhibited tumor necrosis factor α and interleukin-12 (IL-12) release, whereas it enhanced the secretion of IL-10 from mDCs. The use of selective receptor agonists revealed that the modulation of the cytokine and cell-surface marker profile was due to activation of A2 adenosine receptor. Functionally, adenosine reduced the allostimulatory capacity of iDCs, but not of mDCs. More important, DCs matured in the presence of adenosine had a reduced capacity to induce T helper 1 (Th1) polarization of naive CD4+ T lymphocytes. Finally, adenosine augmented the release of the chemokine CCL17 and inhibited CXCL10 production by mDCs. In aggregate, the results provide initial evidence that adenosine diminishes the capacity of DCs to initiate and amplify Th1 immune responses.</jats:p>

収録刊行物

  • Blood

    Blood 101 (10), 3985-3990, 2003-05-15

    American Society of Hematology

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