Localization and characterization of [¹²⁵I]iodomelatonin binding sites in duck gonads

• Elizabeth A. Ayre

• Zheng Pin Wang

• Gregory M. Brown

• Shiu Fun Pang

抄録

<jats:label /> Ayre EA, Wang ZP, Brown GM, Pang SF. Localization and characterization of [ 125 I]iodomelatonin binding sites in duck gonads. J. Pineal Res. 1994: 17: 39–47. The characterization and localization of [ 125 I]iodomelatonin binding sites in the gonads advances the understanding of possible regulatory sites of melatonin action. With the availability of [ 125 I]iodomelatonin as a biologically active radioligand, our study utilized a combined approach of autoradiography for anatomical resolution together with an established radioligand binding assay to assess mid‐light [ 125 I]iodomelatonin binding in the testes and ovaries of ducks. In the autoradiography study, specific [ 125 I]iodomelatonin binding was shown to be homogeneous throughout the testes, while in the ovaries, specific [ 125 I]iodomelatonin binding appeared to be concentrated around the follicle. Radioligand binding assay results indicated a single class of binding sites with the maximum number of [ 125 I]iodomelatonin binding sites measured at 1. 91 ± 0. 70 fmol/mg protein in testicular membrane and 4. 54 ± 0. 64 fmol/mg protein in ovarian membrane. [ 125 I]Iodomelatonin binding affinity, characterized by equilibrium dissociation constants of 29 ± 6 pmol/L in testicular membrane and 53 ± 9 pmol/L in ovarian membrane, was in accordance with circulating melatonin levels, suggesting an appropriate concentration for eliciting a physiological response. [I]Iodomelatonin binding in duck gonads satisfied all the criteria for a binding site, being rapid, stable, saturable, reversible, specific, and of high affinity, and may indicate a direct pineal‐gonadal connection.

収録刊行物

• Journal of Pineal Research

  Journal of Pineal Research 17 (1), 39-47, 1994-08

  Wiley

キーワード

• Endocrinology

詳細情報

• CRID: 1360292618875199616
• NII論文ID: 30025323586
• DOI: 10.1111/j.1600-079x.1994.tb00112.x
• ISSN: 1600079X
• ISSN: 07423098
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-079X.1994.tb00112.x
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-079X.1994.tb00112.x
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